Composite

Part:BBa_K1030004:Design

Designed by: Wei Huang   Group: iGEM13_SUSTC-Shenzhen-A   (2013-09-16)


J23104->tetA-asv+sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1106
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 960
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 406
    Illegal NgoMIV site found at 566
    Illegal NgoMIV site found at 934
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1273


Design Notes

We use a series of synthetic constitutive promoter to tune the expression of tetA, which in turn tune the extracellular Nickel tolerance level. sfGFP is used to monitor tetA expression.


Source

J23104 and sfGFP are from iGEM, tetA is from pBR322.

References